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...difference in acetone precipitation and ammonium...

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Question by jeni
Submitted on 2/23/2004
Related FAQ: Sci.chem FAQ - Part 1 of 7
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what is the difference in acetone precipitation and ammonium sulphate precipitation for protein purification, and which is effective and why?



Answer by annu
Submitted on 8/12/2004
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why cold acetone is used in acetone precipitation of protein

 

Answer by pi
Submitted on 5/5/2005
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i do not know

 

Answer by ice
Submitted on 5/7/2005
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acetone precipitation will denature the protein which could be used for the last step of purification to concentrate the samples.

ammonium sulphate precipitation is commonly used for the first step of protein purification, and the protein will keep an active form after stepwise dialysis.

 

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